PEI Prime™ Performance Data (HEK293)

PEI Prime™ is an exceptional, high-performance transfection reagent for HEK293 suspension cultures. Transient gene expression (TGE) using PEI Prime™ shows expression levels significantly higher (48%) than the leading competitor PEI.

Method: 20 mL cultures containing HEK293 suspensions were transfected with a CMV-SEAP plasmid at optimized PEI/DNA ratios using either PEI Prime™ (3:1 here) or leading competitor PEI. SEAP expression levels were quantified 5 days post-transfection using phosphatase reporter dye and UV/Vis. Values are reported as percentage of leading competitor PEI.