PEI Prime™ Performance Data (CHO)

PEI Prime™ is an excellent reagent for CHO suspension cultures. Multiple SEAP reporter assays show comparable performance between PEI Prime™ and leading competitor PEI in CHO suspension systems.

Method: 20 mL cultures containing CHO suspensions were transfected with a CMV-SEAP plasmid at optimized PEI/DNA ratios using either PEI Prime™ (5:1 here) or leading competitor PEI. SEAP expression levels were quantified 5 days post-transfection using phosphatase reporter dye and UV/Vis. Values are reported as percentage of leading competitor PEI.